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Biotin takes part in the formation of the protein structures of hair and nails at the cellular level, but this does not mean an immediate effect. Hair grows about 1–1.5 cm a month, and nails even more slowly. Any changes related to their structure become noticeable only as new tissue grows out.
In practice this means that it makes sense to assess the result no sooner than after several weeks of regular use, and in some cases after two or three months or more. This is a general feature of supplements that affect quickly renewing tissues: it takes time for new cells to complete a full growth cycle.
In addition, the condition of hair and nails is influenced by many factors besides biotin: overall diet, stress level, hormonal balance, care. The supplement can be part of a comprehensive approach, but it does not replace a balanced diet and other aspects of lifestyle.
If you notice no changes after a long course, or want to understand the causes of problems with your hair and nails, it is better to see a specialist — sometimes these symptoms are caused by other factors that need separate attention.